Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown
نویسندگان
چکیده
منابع مشابه
Flexible isoform-level differential expression analysis with Ballgown
We have built a statistical package called Ballgown for estimating differential expression of genes, transcripts, or exons from RNA sequencing experiments. Ballgown is designed to work with the popular Cufflinks transcript assembly software and uses well-motivated statistical methods to provide estimates of changes in expression. It permits statistical analysis at the transcript level for a wid...
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RNA-seq is currently the technology of choice for global measurement of transcript abundances in cells. Despite its successes, isoform-level quantification remains difficult because short RNA-seq reads are often compatible with multiple alternatively spliced isoforms. Existing methods rely heavily on uniquely mapping reads, which are not available for numerous isoforms that lack regions of uniq...
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ژورنال
عنوان ژورنال: Nature Protocols
سال: 2016
ISSN: 1754-2189,1750-2799
DOI: 10.1038/nprot.2016.095